Near-Infrared Solutions for Multiplexed Confocal Imaging FV3000 Red System
Interested in imaging with far-red fluorescent probes? Expand your multiplex capabilities with near-infrared (NIR) modules for the FV3000 microscope, including a 730 nm or 785 nm diode laser and GaAs PMTs for high-sensitivity detection up to 890 nm.
- Greater multiplexing capabilities
- Reduced autofluorescence
- Gentler live-cell imaging
- Deeper imaging with less absorption and scattering
NIR Laser Diodes
- Less cross-talk when selecting dyes
- Laser diodes (LD730/785) designed to be driven in continuous wave (CW) mode, offering stability, low maintenance, and a long lifetime
Applicable dyes for NIR imaging
| Laser | Fluorescent dye | λ_Ex (nm) | λ_Em (nm) |
|---|---|---|---|
| LD730 | ATTO 725 | 727 | 752 |
| Cy7 | 743 | 767 | |
| ATTO 740 | 743 | 763 | |
| DiR | 748 | 780 | |
| DyLight 750 | 751 | 772 | |
| Alexa Fluor 750 | 752 | 779 | |
| LD785 | IRDye 800RS | 767 | 785 |
| DyLight 800 | 770 | 795 | |
| IRDye 800CW | 778 | 794 | |
| Alexa Fluor 790 | 782 | 805 | |
| Cy7.5 | 790 | 810 |
Cooled GaAs PMT Detector for NIR Imaging
- Significantly extends the detection range in the near-infrared
- High sensitivity up to 890 nm
- One or two GaAs PMT detectors can be combined with GaAsP or standard PMT detectors, enabling up to six individual detectors for multiplex imaging from the VIS to NIR
- Efficient cooling reduces thermal noise emitted from the photocathode for a high signal-to-noise ratio at extremely low light levels
High Transmittance Optics for NIR Imaging
High transmittance from VIS to IR with silver-coated scan mirrors and Olympus’ unique 1600 Coating on the scan lens.
Deep Tissue Observation with Silicone Oil Immersion Objectives in the NIR
- NIR fluorescence works well for deep imaging because NIR light scatters less in live tissue
- The refractive index of silicone oil (ne≈1.40) is close to that of living tissue (ne≈1.38), enabling high-resolution observations deep inside living tissue with minimal spherical aberration
- The UPLSAPO30XIR (NA 1.05, WD 800 μm) offers higher NIR transmittance thanks to Olympus’ 1600 Coating
Multiplexing with X Line High-Performance Objectives
- Greater multiplexing imaging is possible using additional NIR detection channels
- Reduced chromatic aberration during co-localization analysis (corrected at 400 nm–1000 nm)
- Higher numerical aperture, excellent image flatness, and high transmittance from UV to NIR
TruFocus Red Z-Drift Compensator
- Uses a near-infrared laser to locate the coverslip interface and corresponding focal position
- Near-infrared light minimizes cell damage during prolonged observation
- Works well for live cell imaging with far-red and near-infrared fluorophores
For more information contact us.